20 February 2017 Run – JGK Lab AfriQA

Scheme reference	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Scheme reference	Type	Identification (as per the labels)	Storage	Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
JGKPT01 Antimicrobial susceptibility	Animal sp.: Cattle 2 FD cultures (clinical pathogens)	ATB01C – ATB02C	2-8^oC	Add 1 ml sterile DH₂O to the vial	Test immediately after reconstitution Observe deadline for submission of results	Disc diffusion technique	R.E.F 1.2 (supplied)	Zone Ø in millimeters (Results: S, I, R)	Culture: 1 Susceptibility: Supplied discs: 3 Own discs: 1
JGKPT01 Antimicrobial susceptibility	Antimicrobial discs (x18)	Ceftiofur 30 µg (x6) Spectinomycin 100 µg (x6) Chloramphenicol 30 µg (x6)	2-8^oC	Discs are ready to use (Commercial discs)		Disc diffusion technique	R.E.F 1.2 (supplied)	Zone Ø in millimeters (Results: S, I, R)
JGKPT03 Brucella: RBT	6 FD bovine sera	RBT01 – RBT06	2-8^oC	Add 250 µl sterile DH₂O to the vial	Test within 5 days after reconstitution Observe deadline for submission of results	RBT	R.E.F 3.5 (supplied)	Positive/Negative	1
JGKPT04 Brucella: CFT	6 FD bovine sera	CFT01 – CFT06	2-8^oC	Add 250 µl sterile DH₂O to the vial	Test within 5 days after reconstitution Observe deadline for submission of results	CFT (DAFF approved SOP for SA Labs)	R.E.F 3.5 (supplied)	CFTIU/ml	2
JGKPT05 Brucella: MRT	3 Preserved cow’s milk	CAM01 – CAM03	2-8^oC	NA Ready to process	Preferably immediately but no later than 5 days after dispatch	MRT	R.E.F 3.5 (supplied)	Positive/Negative	1
JGKPT06 Brucella: Culture & Identification	3 FD cultures	CAC01 – CAC03	2-8^oC	Add 1 ml sterile DH₂O to the vial	Test immediately after reconstitution Observe deadline for submission of results	Conventional microbiology and Molecular technique	R.E.F 06 (supplied)	Presence/Absence: Brucella abortus Strain: Field or vaccine	1
JGKPT61-64 Trichomonas and Campylobacter: Culture/PCR	5 Cultures in transport media	SW01 – SW05	2-8^oC	NA Ready to process	Preferably immediately but no later than 5 days after dispatch	Conventional microbiology and Molecular technique	R.E.F 61.4 (supplied)	Presence/Absence: Trichomonas foetus, Campylobacter fetus	1
JGKPT61 & 62 Trichomonas only: Culture/PCR	3 Cultures in transport media	TRI01 – TRI03	2-8^oC	NA Ready to process	Preferably immediately but no later than 5 days after dispatch	Conventional microbiology and Molecular technique	R.E.F 61.4 (supplied)	Presence/Absence: Trichomonas foetus	1
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+ Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on or before the 13^th of March 2017. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None