PT Instructions
6 March 2017 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe |
Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type |
Identification (as per the labels) |
Storage | |||||||
|
Antibiogram – mastitis pathogens JGKPT02 |
2 FD cultures, 18 ATB discs |
ATB01M – ATB02M Discs: Ticarcillin/Clavulanic acid, Ampicillin, Trimethoprim/Sulfamethoxazole |
2-8oC |
Add 1 ml sterile DH2O to the vial Mix well and test |
Test within 2 days after reconstitution Observe deadline for submission of results |
Kirby-Bauer Disc diffusion |
R.E.F 02 (supplied) |
Zone Ø in millimeters (Results: S, I, R) |
Culture: 1 Susceptibility: Supplied discs: 3 Own discs: 1 |
|
Antimicrobial residues – meat (β-lactams OR Tetracyclines) JGKPT32, 33 |
2 pieces of meat (25 g) | RES01F – RES02F | 2-8oC | Ready to process |
Test within 5 days after dispatch Observe deadline for submission of results |
Rapid test Microbiology Chromatography |
R.E.F 32-44 (supplied) |
Presence/Absence | 1 |
|
Antimicrobial residues – meat (nonspecific) JGKPT34 |
4 pieces of meat (25 g) | RES01F – RES04F | 2-8oC | Ready to process |
Test within 5 days after dispatch Observe deadline for submission of results |
Rapid test Microbiology Chromatography |
R.E.F 32.44 (supplied) |
Presence/Absence | 1 |
|
Antimicrobial residues – milk (β-lactams, Tetra, nonspecific) JGKPT29, 30, 31 |
4 FD milk | RES01M – RES04M | 2-8oC |
Add 10 ml sterile DH2O to the vial Mix well and test |
Test within 5 days after dispatch Observe deadline for submission of results |
Rapid test Microbiology Chromatography |
R.E.F 26-31 (supplied) |
Presence/Absence | 1 |
|
Avian influenza virus (Virus isolation: H6) JGKPT76 |
Frozen virus suspension | AIVI01 – AIVI03 | -86oC | Ready to process |
Long term if kept frozen Observe deadline for submission of results |
Virus propagation in EE |
R.E.F 76.9 (supplied) |
Presence/Absence | 1 |
|
Avian influenza – serum (H5/H6/H7) JGKPT69 |
6 FD sera | AI01 – AI06 | 2-8oC |
Add 350 µl sterile DH2O to the vial Stir and test |
Test within 5 days after reconstitution Observe deadline for submission of results |
ELISA & HA/HI test |
R.E.F 65-75 (supplied) |
ELISA: OD HI: Titres |
ELISA: 2 HI: 2 |
|
Bovine viral diarrhoea – serum JGKPT65 |
5 liquid sera | BVD01 – BVD05 | 2-8oC | Ready to process |
Test within 5 days after dispatch Observe deadline for submission of results |
ELISA |
R.E.F 65-75 (supplied) |
OD | 2 |
|
Cattle diseases scenario JGKPT20 |
3 FD cultures | BAC01C – BAC03C | 2-8oC |
Add 1 ml sterile DH2O to the vial Mix well and test |
Test within 2 days after reconstitution Observe deadline for submission of results |
Culture & ID |
R.E.F 20.5 (supplied) |
Target bacteria | 1 |
|
Chickens diseases scenario JGKPT21 |
3 FD cultures | BAC01CH – BAC03CH | 2-8oC |
Add 1 ml sterile DH2O to the vial Mix well and test |
Test within 2 days after reconstitution Observe deadline for submission of results |
Culture & ID |
R.E.F 20.5 (supplied) |
Target bacteria | 1 |
|
Coliforms – meat (Enumeration) JGKPT36 |
2 FD cultures | COL01F – COL02F | 2-8oC |
Add 1 ml sterile DH2O to the vial Mix well and test |
Test immediately after reconstitution Observe deadline for submission of results |
Microbiology (conventional & automated) |
R.E.F 32-44 (supplied) |
Counts |
1 (averaged) |
|
E. coli O157 – meat (Detection) JGKPT37 |
3 pieces of meat (25 g) | FH01 – FH03 | 2-8oC | Ready to process |
Test within 5 days after dispatch Observe deadline for submission of results |
Microbiology (conventional & automated) |
R.E.F 32-44 (supplied) |
Presence/Absence | 1 |
|
Fasciola hepatica – serum JGKPT67 |
5 FD sera | FAS01 – FAS05 | 2-8oC | Add 500 µl sterile DH2O to the vial |
Test within 5 days after reconstitution Observe deadline for submission of results |
ELISA |
R.E.F 65-75 (supplied) |
OD | 2 |
|
Infectious bronchitis virus – serum JGKPT71 |
6 FD sera | IBV01 – IBV06 | 2-8oC |
Add 250 µl sterile DH2O to the vial Stir and test |
Test within 5 days after reconstitution Observe deadline for submission of results |
ELISA |
R.E.F 65-75 (supplied) |
OD | 2 |
|
Listeria monocytogenes – meat (Detection) JGKPT42 |
3 pieces of meat (25 g) | FH01 – FH03 | 2-8oC | Ready to process |
Test within 5 days after dispatch Observe deadline for submission of results |
Microbiology (conventional & automated) |
R.E.F 32-44 (supplied) |
Presence/Absence | 1 |
|
Mastitis pathogens – milk JGKPT60 |
2 FD cultures | MAST01 – MAST02 | 2-8oC |
Add 1 ml sterile DH2O to the vial Mix well and test |
Test within 2 days after reconstitution Observe deadline for submission of results |
Culture & ID |
R.E.F 59-60 (supplied) |
Target bacteria | 1 |
|
Milk hygiene: Coliforms count JGKPT27 |
3 FD milk (10 ml) |
MHCOL01 – MHCOL03 | 2-8oC |
Instructions will follow by email |
Test immediately after reconstitution Observe deadline for submission of results |
3M Petri Films | R.E.F 26-31 | Counts | 2 |
|
Milk hygiene: Total bacteria count JGKPT26 |
3 FD milk (10 ml) |
MHTBC01 – MHTBC03 | 2-8oC |
Instructions will follow by email |
Test immediately after reconstitution Observe deadline for submission of results |
3M Petri Films |
R.E.F 26-31 (supplied) |
Counts | 2 |
|
Milk hygiene: E. coli count JGKPT28 |
3 FD milk (10 ml) |
MHEC01 – MHEC03 | 2-8oC |
Instructions will follow by email |
Test immediately after reconstitution Observe deadline for submission of results |
3M Petri Films |
R.E.F 26-31 (supplied) |
Counts | 2 |
|
Mycoplasma gallisepticum – serum – chicken JGKPT74 |
6 FD sera | MG01 – MG06 | 2-8oC |
Add 250 µl sterile DH2O to the vial Stir and test |
Test within 5 days after reconstitution Observe deadline for submission of results |
RSPA/ELISA |
R.E.F 26-31 (supplied) |
RSPA: Positive/Negative ELISA: OD |
RSPA: 1 ELISA: 2 |
|
Newcastle disease – serum JGKPT72 |
6 FD sera | NDV01 – NDV06 | 2-8oC |
Add 250 µl sterile DH2O to the vial Stir and test |
Test within 5 days after reconstitution Observe deadline for submission of results |
ELISA & HA/HI test |
R.E.F 65-75 (supplied) |
ELISA: OD HI: Titres |
ELISA: 2 HI: 2 |
|
Newcastle disease virus (Virus isolation) JGKPT78 |
Frozen virus suspension | NDVI01 – NDVI06 | -86oC | Ready to process |
Long term if kept frozen Observe deadline for submission of results |
Virus propagation in EE |
R.E.F 76.9 (supplied) |
Positive/Negative | 1 |
|
Salmonella enteritidis – serum JGKPT73 |
6 FD sera | SE01 – SE06 | 2-8oC |
Add 250 µl sterile DH2O to the vial Stir and test |
Test within 5 days after reconstitution Observe deadline for submission of results |
RSPA/ELISA |
R.E.F 65-75 (supplied) |
RSPA: Positive/Negative ELISA: OD |
RSPA: 1 ELISA: 2 |
|
Salmonella species – meat JGKPT43 |
3 pieces of meat (25 g) | FH01 – FH03 | 2-8oC | Ready to process |
Test within 5 days after dispatch Observe deadline for submission of results |
Microbiology (conventional & automated) |
R.E.F 32-44 (supplied) |
Presence/Absence | 1 |
|
Somatic cells counts – milk JGKPT59 |
6 Milk – liquid (20 ml) |
SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing |
Test within 5 days after dispatch Observe deadline for submission of results |
Cytology |
R.E.F 59-60 (supplied) |
Counts | 3 |
|
Total viable count – meat (Enumeration) JGKPT44 |
2 FD cultures | TVC01 – TVC02 | 2-8oC |
Add 1ml sterile DH2O to the vial Stir and test |
Test immediately after reconstitution Observe deadline for submission of results |
Microbiology (conventional & automated) |
R.E.F 32-44 (supplied) |
Counts |
1 (averaged) |
|
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT76 and JGKPT78 (Virus isolation) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 27th of March 2017. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
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FD: Freeze-Dried