WEEK 43: 17 & 18 OCTOBER 2022 RUN – JGK Lab AfriQA

Scheme reference	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
	Type	Identification (as per the labels)	Storage
JGKPT01 Antimicrobial susceptibility (Clin. Pathogens)	2 FD Cultures (main samples for the tests) 1 NA plate – BKP sample (use BKP sample if you encounter a problem) + 2 clean MH agar plates	ATB01/C – pig ATB02/C – horse	2-8^oC	ATB01: Add 400 µl sD.H₂O ATB02: Add 500 µl sD.H₂O Supplied Mueller Hinton ATB01 susceptibility test must be conducted using the supplied MH agar plates	Once reconstituted, samples must be tested without delay	Disc diffusion technique (Kirby Bauer)	R.E.F 1.0 (available on www.jgklab.co.za)	Zone Ø in millimeters. Results: Sensitive (S), Intermediate (I), Resistance (R)	Culture ID: 1 Susceptibility: Supplied discs: 3 (as per result sheet)
	Antimicrobial discs (x18)	Cephazolin 30 µg (x6) Apramycin 15 µg (x6) Sulphonamides 300 µg (x6)		Discs are ready to use (Commercial discs)
JGKPT03 Brucella: RBT (Samples for labs doing RBT only)	6 FD cattle sera 1 PC (lot 22). 0.5 ml 1 NC (CANEG) 1 ml	RBT01 – RBT06	2-8^oC	Samples 1, 2, and the PC: Add 0.5 ml sDH₂O to the vial Samples 3, 4, 5, 6 and the NC Add 1 ml sDH₂O to the vial	Once reconstituted, samples must be tested without delay	RBT only	R.E.F 3-5 (available on www.jgklab.co.za)	Positive/Negative Positive results must be scored 1+ to 3+	1
JGKPT04 Brucella: RBT/CFT (Samples for labs doing both RBT and CFT)	6 FD cattle sera 1 PC (lot T183) 1 ml 1 NC (CANEG) 1 ml	RBT/CFT01 – RBT/CFT06	2-8^oC	Samples 1, 2, 5, 6, PC, NC: Add 1 ml sDH₂O to the vial Samples 3 and 4: Add 0.5 ml sDH₂O to the vial Positive control: Use the PC to test for analytical sensitivity as follows: (1) Reconstitution with 1 ml sDH₂O, (2) Two-fold serial dilution in a microtiter plate (must be dilution to extinction), (3) Test both RBT and CFT at each dilution point/factor	Once reconstituted, samples must be tested without delay	RBT and CFT	R.E.F 3-5 (available on www.jgklab.co.za)	RBT: Positive/Negative Positive results must be scored 1+ to 3+	1
								CFT: CFTIU/ml Positive control: RBT: report the highest dilution that produced a negative result (e.g. 1/8) CFT: report the lowest titer and the dilution point where it occurred (e.g. 15.8; 15 = titer; 8 = 1/8 i.e. the highest dilution where 15 was quantified	2 1 1
JGKPT05 Brucella: MRT	4 bulk tank milk	CAM01 – CAM04	2-8^oC	NA Ready to process	Test immediately upon receipt	MRT	R.E.F 3-5 (available on www.jgklab.co.za)	Positive/Negative	1
JGKPT06 Brucella: Culture & Identification	Sample 1: swab (genital discharge) samples 2, 3: FD culture Sample 4: cow’s milk	CAC01 – CAC04	2-8^oC	Sample 1: Ready to process Sample 2: Add 300 µL sD.H₂O to the vial Sample 3: Add 500 µL sD.H₂O to the vial Sample 4: Ready to process	Once reconstituted, samples must be tested without delay	Conventional microbiology and Molecular technique	R.E.F 06 (available on www.jgklab.co.za)	Presence/Absence: Brucella abortus Strain: Field or vaccine	1
JGKPT07 Brucella Slides	6 unstained smears	Slide 1: x 2 Slide 2: x 2 Slide 3: x 2	Room T^o Dust-free	Proceed with staining technique as per Lab SOP	When and as convenient, but before submission deadline	Stamp’s staining technique	R.E.F 06 (available on www.jgklab.co.za)	Positive/Negative for Brucella	1
JGKPT15 Bacteriology: PIG	3 x Amies swab (main samples for the test) 1 x NA plate as BKP sample NB: The NA plate has only 2 grown cultures (1 and 2). No BKP for sample 3. Culture it directly from the swab	BAC01/P – BAC03/P (Pig 01, Pig 02, Pig 03)	2-8^oC	N/A Proceed with culture / subculture	Test immediately upon receipt	Conventional microbiology and Molecular technique	R.E.F 11-16 (available on www.jgklab.co.za)	Full ID of all bacteria isolated from each sample	1
JGKPT16 Bacteriology: SHEEP & GOATS	3 FD Cultures	BAC01/SG – BAC03/SG (Sheep/Goat 01 – Sheep/Goat 03)	2-8^oC	Sample 1: Add 350 µL sD.H₂O to the vial Sample 2: Add 300 µL sD.H₂O to the vial Sample 3: Add 500 µL sD.H₂O to the vial	Once reconstituted, samples must be tested without delay
JGKPT35 & 37 Trichomonas and Campylobacter ID by Culture	5 Cultures in transport media	SW01 – SW05	2-8^oC	NA Ready to process	Test immediately upon receipt	Conventional microbiology	R.E.F 35-38 (available on www.jgklab.co.za)	Presence/Absence: Trichomonas foetus, Campylobacter fetus	1
JGKPT36 & 38 Trichomonas and Campylobacter ID by PCR						Molecular technique		Presence/Absence: Trichomonas foetus	1
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+ Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The R.E.F grouped into WK8,19,31,43 and WK9,20,32,44 are available online (www.jgklab.co.za) and can be downloaded under “PUBLICATIONS” The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on Wednesday, 2^nd of November 2022. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None