PT Instructions
04 May 2022 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme
reference |
Samples | Samples reconstitution | Testing timeframe | Approved test methods | Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| JGKPT01
Antimicrobial susceptibility (Clin. Pathogens) |
2 Cultures on NA plates
2 Backup cultures on Amies swabs |
ATB01/C – chicken
ATB02/C – chicken |
2-8oC | N/A
Proceed with culture / subculture |
Preferably, test immediately but no later than 48 hours from date of dispatch | Disc diffusion technique
(Kirby Bauer) |
R.E.F 1.0
(available on www.jgklab.co.za) |
Zone Ø in millimeters.
Results: Sensitive (S), Intermediate (I), Resistance (R) |
Culture ID: 1
Susceptibility: Supplied discs: 3 (as per result sheet) |
| Antimicrobial discs (x18) | Timilcosin 15 µg (x6)
Sulphafurazole 300 µg (x6) Imipenem 10 µg (x6) |
Discs are ready to use (Commercial discs) | |||||||
| JGKPT03
Brucella: RBT (Samples for labs doing RBT only) |
6 FD cattle sera
1 PC (13103). 0.5 ml 1 NC (14102). 0.5 ml |
RBT01 – RBT06 | 2-8oC | Add 0.5 ml sterile DH2O to each vial | Once reconstituted, samples must be tested w/o delay | RBT only | R.E.F 3-5
(available on www.jgklab.co.za) |
Positive/Negative
Positive results must be scored 1+ to 3+ |
1 |
| JGKPT04
Brucella: RBT/CFT (Samples for labs doing both RBT and CFT) |
6 FD cattle sera
1 PC (1164). 1 ml 1 NC (121218). 1 ml |
RBT/CFT01 – RBT/CFT06 | 2-8oC | Samples 1, 2, 3:
Add 1 ml sterile DH2O to each vial Samples 4, 5, 6: Add 0.5 ml sterile DH2O to each vial |
Once reconstituted, samples must be tested without delay | RBT and CFT | R.E.F 3-5
(available on www.jgklab.co.za) |
RBT: Positive/Negative
Positive results must be scored 1+ to 3+ |
1 |
| CFT:
CFTIU/ml |
2 | ||||||||
| JGKPT05
Brucella: MRT |
4 bulk tank milk | CAM01 – CAM04 | 2-8oC | NA
Ready to process |
Preferably, test immediately but no later than 48 hours from date of dispatch | MRT | R.E.F 3-5
(available on www.jgklab.co.za) |
Positive/Negative | 1 |
| JGKPT06
Brucella: Culture & Identification |
Sample 1: cow’s milk
Samples 2 and 3: FD cultures |
CAC01 – CAC03 | 2-8oC | Sample 1: ready to use
Samples 2 and 3: add 0.5 ml sterile DH2O to each vial |
Once reconstituted, samples must be tested without delay | Conventional microbiology and
Molecular technique |
R.E.F 06
(available on www.jgklab.co.za) |
Presence/Absence: Brucella abortus
Strain: Field or vaccine |
1 |
| JGKPT07
Brucella Slides |
9 unstained smears | Slide 1: x 3
Slide 2: x 3 Slide 3: x 3 |
Room To
Dust-free |
Proceed with staining technique as per Lab SOP | When and as convenient, but before submission deadline | Stamp’s staining technique | R.E.F 06
(available on www.jgklab.co.za) |
Positive/Negative for Brucella | 1 |
| JGKPT11
Bacteriology: CATTLE |
3 Cultures on agar plates
1 Broth 4 Backup cultures on Amies swabs |
BAC01/C, BAC02/C, BAC03/C: 3 cultures on NA plate
BAC04/C: broth in a vial BAC01/C – BAC04/C: backup cultures on Amies swabs |
2-8oC | N/A
Proceed with culture / subculture |
Preferably, test immediately but no later than 48 hours from date of dispatch | Conventional microbiology and
Molecular technique |
R.E.F 11-16
(available on www.jgklab.co.za) |
Full ID of all bacteria isolated from each sample | 1 |
| JGKPT12
Bacteriology: CHICKENS |
3 Cultures on agar plates
3 Backup cultures on Amies swabs |
BAC01/CH, BAC02/CH, BAC03/CH: 3 cultures on NA plate
BAC01/CH – BAC03/CH: backup cultures on Amies swabs |
2-8oC | N/A
Proceed with culture / subculture |
Preferably, test immediately but no later than 48 hours from date of dispatch | ||||
| JGKPT35 & 37
Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 5 days from date of dispatch | Conventional microbiology | R.E.F 35-38
(available on www.jgklab.co.za) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 |
| JGKPT36 & 38
Trichomonas and Campylobacter ID by PCR |
Molecular technique | Presence/Absence: Trichomonas foetus | 1 | ||||||
|
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The R.E.F grouped into WK8,19,31,43 and WK9,20,32,44 are available online (www.jgklab.co.za) and can be downloaded under “PUBLICATIONS” The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on Wednesday, 18th of May 2022. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
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FD: Freeze-Dried