18 October 2021 Run – JGK Lab AfriQA

Scheme reference	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Scheme reference	Type	Identification (as per the labels)	Storage	Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
JGKPT01 Antimicrobial susceptibility (clin. pathogens)	2 Cultures on 1 x Agar plate	ATB01/C – cattle ATB02/C – dog	2-8^oC	NA Ready to process	Preferably, test immediately upon receipt of the samples	Disc diffusion technique (Kirby Bauer)	R.E.F 1.0 (available on www.jgklab.co.za)	Zone Ø in millimeters. Results: Sensitive (S), Intermediate (I), Resistance (R)	Culture ID: 1 Susceptibility: Supplied discs: 3 (as per result sheet)
	Antimicrobial discs (x18)	Apramycin 15 µg (x6) Compound sulfonamides 300 µg (x6) Gentamycin 10 µg (x6)	2-8^oC	Discs are ready to use (Commercial discs)	Preferably, test immediately upon receipt of the samples	Disc diffusion technique (Kirby Bauer)	R.E.F 1.0 (available on www.jgklab.co.za)
JGKPT03 Brucella: RBT	6 FD cattle sera (samples for Labs testing for RBT only)	RBT01 – RBT06	2-8^oC	Add 0.5 ml sterile DH₂O to each vial	Test immediately after reconstitution of the samples before deadline for submission of results	RBT	R.E.F 3-5 (available on www.jgklab.co.za)	Positive/Negative Agglutination score for positive results (1+ to 3+)	1
JGKPT04 Brucella: CFT	6 FD cattle sera (samples for Labs testing for both RBT and CFT)	RBT/CFT01 – RBT/CFT06	2-8^oC	Add 1 ml sterile DH₂O to each vial	Test immediately after reconstitution of the samples before deadline for submission of results	RBT CFT (DAFF approved SOP for SA Labs)	R.E.F 3-5 (available on www.jgklab.co.za)	RBT: Positive/Negative, agglutination score for positive results (1+ to 3+) CFT: CFTIU/ml	RBT: 1 CFT: 2
JGKPT05 Brucella: MRT	4 bulk tank milk	CAM01 – CAM04	2-8^oC	NA Ready to process	Preferably, test immediately but no later than 48 hours from date of dispatch	MRT	R.E.F 3-5 (available on www.jgklab.co.za)	Positive/Negative	1
JGKPT06 Brucella: Culture & Identification	2 FD cultures 1 cow’s milk 1 Swab	CAC01 – CAC02 CAC03 CAC04	2-8^oC	FD: Add 0.5 ml sterile DH₂O to CAC01 & CAC02 Milk and Swab: Ready to process	FD: Test immediately after reconstitution. Milk and Swab: Test immediately upon receipt of the samples	Conventional microbiology and Molecular technique	R.E.F 06 (available on www.jgklab.co.za)	Brucella abortus: Presence/Absence Strain of BA isolated: Field or vaccine	1
JGKPT07 Brucella Slides	3 unstained smears	Slide 1: x 3 Slide 2: x 3 Slide 3: x 3	Room T^o Dust-free	Proceed with staining technique as per Lab SOP	When and as convenient, but before submission deadline	Stamp’s staining technique	R.E.F 06 (available on www.jgklab.co.za)	Positive/Negative for Brucella	1
JGKPT35 & 37 Trichomonas and Campylobacter ID by Culture	5 Cultures in transport media	SW01 – SW05	2-8^oC	NA Ready to process	Preferably, test immediately but no later than 72 hours from date of dispatch	Conventional microbiology	R.E.F 35-38 (available on www.jgklab.co.za)	Presence/Absence: Trichomonas foetus, Campylobacter fetus	1
JGKPT36 & 38 Trichomonas and Campylobacter ID by PCR	5 Cultures in transport media	SW01 – SW05	2-8^oC	NA Ready to process		Molecular technique	R.E.F 35-38 (available on www.jgklab.co.za)	Presence/Absence: Trichomonas foetus	1
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+ Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on Wednesday, 3^rd of November 2021. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None