PT Instructions

10 May 2021 Run

PURPOSE

To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1

INSTRUCTIONS

Scheme Description and Code Samples Samples  reconstitution Testing timeframe Approved test methods

 

Sheet to be used for submission of results to JGK Unit of measurement (Results) Number of test replicates per sample
Type Identification

(as per the labels)

Storage
Antibiogram – mastitis pathogens

JGKPT02

2 x FD ATB01M – ATB02M 2-8oC Add 300 µL sterile DH2O to each vial Culturing of bacteria should be done Immediately upon reconstitution Culturing: micro

Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative)

R.E.F 02

(supplied)

Zone Ø in millimeters

(Results: S, I, R)

Culture: 1 rep

Susceptibility:

3 replicates (supplied discs)

18 x antibiotic discs 6 x Neomycin 10 µg

6 x Penicillin 10 IU

6 x Oxacillin 5 µg

Ready to process
Antimicrobial residues – meat

JGKPT23-25

4 x pieces of meat RES-01-F – RES-04-F 2-8oC Ready to process Test immediately upon receipt Rapid test

Microbiology

Chromatography

R.E.F 23-25

(supplied)

Presence/Absence of antimicrobials

ID of the antibiotic

1 rep
Antimicrobial residues – milk

JGKPT20-22

4 x FD milk RES-01-M – RES-04-M 2-8oC Add 3 ml sterile DH2O to each vial Test immediately upon receipt/reconstitution Rapid test

Microbiology

Chromatography

R.E.F 20-22

(supplied)

Presence/Absence of antimicrobials

ID of the antibiotic

1 rep
Food hygiene – meat (Detection)

JGKPT25-30

4 x FD FH01 – FH04 2-8oC Add 350 µL sterile DH2O to each vial Test immediately upon reconstitution Microbiology (conventional & R.E.F 25-30

(supplied)

Presence/Absence of target bacteria 1 rep
Mastitis pathogens – milk

JGKPT34

2 x FD MAST01 – MAST02 2-8oC Add 300 µL sterile DH2O to each vial Test immediately upon reconstitution Culture & ID R.E.F 33-34

(supplied)

Target bacteria 1 rep
Milk hygiene: TBC, Coliforms and E. coli counts

JGKPT17-19

4 x FD milk

(10 ml)

MH01 – MH04 2-8oC Add 10 ml sterile DH2O to each vial. Vortex /mix thoroughly before testing

N.B. No further dilution should be done

Test immediately upon receipt.

Let the reconstituted samples adjust to room temperature for 30 min before testing

3M Petri Films

Conventional technique

R.E.F 17-19 Counts 2 rep
Aflatoxins & Fumonisins

JGKPT31-32

3 x maize meal

(100 g)

MYC01 – MYC03 2-8oC Ready to process Test immediately upon receipt Chromatography R.E.F 31-32 Presence & quantification (ppb) of Aflatoxins and Fumonisins 1 rep.
BVD & Fasciola

JGKPT39-40

5 x FD bovine sera BVD/FASC01 – BVD/FASC05 2-8oC Add 300 µL sterile DH2O to each vial Test immediately upon reconstitution ELISA R.E.F 39-45 Elisa ODs 2 rep.
Avian influenza - Abs

JGKPT41

6 x FD poultry sera AI01 – AI06 2-8oC Add 500 µL sterile DH2O to each vial Test immediately upon reconstitution ELISA / HI R.E.F 39-45 ODs / Titres 2 rep
Inf. bronchitis virus – Abs

  JGKPT42

6 x FD poultry sera IBV01 – IBV06 2-8oC Add 400 µL sterile DH2O to each vial Test immediately upon reconstitution ELISA / HI R.E.F 39-45 ODs / Titres 2 rep
Newcastle disease – Abs JGKPT43 6 x FD poultry sera NDV01 – NDV06 2-8oC Add 400 µL sterile DH2O to each vial Test immediately upon reconstitution ELISA / HI R.E.F 39-45 ODs / Titres 2 rep
Salmonella enteritidis – Abs

JGKPT44

6 x FD poultry sera SE01 – SE06 2-8oC Add 400 µL sterile DH2O to each vial Test immediately upon reconstitution ELISA / RPAT R.E.F 39-45 Positive / Negative 1 rep
Mycoplasma gallisepticum - Abs

JGKPT45

6 x FD poultry sera MG01 – MG06 2-8oC Add 400 µL sterile DH2O to each vial Test immediately upon reconstitution ELISA / RPAT R.E.F 39-45 Positive / Negative 1 rep
Treatment of samples

No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.

 

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

 

Specific environmental conditions required for the participant to conduct tests

None

 

Results recording

Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

 

Submission of PT results to JGK

All results must be submitted on or before the 26th of May 2021. Any results received after this date will not be included in the analysis.

 

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 

Return of the proficiency test items

None

FD: Freeze-Dried