PT Instructions
25 February 2019 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x FD cultures | ATB01M-ATB02M | 2-8oC | FD Cultures: add 0.5 ml D.H2O to each vial
Discs: ready for use |
Culturing of bacteria should be done Immediately upon reconstitution | Culturing: micro
Susceptibility: Kirby-Bauer / Disc diffusion |
R.E.F 02
(supplied) |
Zone Ø in millimeters
(Results: S, I, R) |
Culture: 1 rep
Susceptibility: 3 replicates (supplied discs) |
| 18 x antibiotic discs | 6 x Neomycin,
6 x Tetracycline, 6 x Pirlimycin |
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| Antimicrobial residues – meat
JGKPT24-26 |
3 x pieces of meat | RES-01-F – RES-03-F | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 24-26
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| Antimicrobial residues – milk
JGKPT21-23 |
4 x milk
(liquid) |
RES-01-M – RES-04-M | 2-8oC | Ready to process | Test immediately upon receipt | Rapid test
Microbiology Chromatography |
R.E.F 21-23
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| Avian influenza virus
(Virus isolation) JGKPT47 |
3 x frozen virus suspension
(1.0 ml) |
AI-VI-01 – AI-VI-03 | -86oC | Ready to process | Long term if kept frozen | Virus propagation in EE | R.E.F 47.50
(supplied) |
Presence/Absence of target virus | 1 rep |
| Avian influenza virus
(PCR) JGKPT48 |
1 x RNA (50 µl)
2 x frozen virus suspension (1.0 ml) |
AI-PCR-01 – AI-PCR-03 | -86oC | Sample 1: RNA, ready to use
Samples 2 & 3: extract as per lab protocol |
Long term if kept frozen | PCR | R.E.F 47.50
(supplied) |
Presence/Absence of matrix & target nucleic acid | 1 rep |
| Food hygiene – meat (Detection)
JGKPT27-31 |
3 x pieces of meat | FH01 – FH03 | 2-8oC | Ready to process | Test immediately upon receipt | Microbiology (conventional & automated) | R.E.F 27-31
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Mastitis pathogens – milk
JGKPT35 |
2 x FD cultures | MAST01 – MAST02 | 2-8oC | Ready to process | Test immediately upon reconstitution | Culture & ID | R.E.F 34-35
(supplied) |
Target bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli count
JGKPT18-20 |
4 FD milk
(10 ml) |
MH01 – MH04 | 2-8oC | Add 10 ml sterile DH2O to each vial,
vortex and test |
Test immediately after reconstitution | 3M Petri Films | R.E.F 18-20 | Counts | 2 rep |
| Newcastle disease virus (Virus isolation)
JGKPT49 |
3 x frozen virus suspensions
(1.0 ml) |
ND-VI-01 – ND-VI-03 | -86oC | Ready to process | Long term if kept frozen | Virus propagation in EE | R.E.F 47-50
(supplied) |
Positive/Negative target virus | 1 rep |
| Newcastle disease virus (PCR)
JGKPT50 |
3 x frozen virus suspensions
(1.2 ml) |
ND-PCR-01 – NDP-CR-03 | -86oC | Ready to process | Long term if kept frozen | PCR | R.E.F 76.9
(supplied) |
Positive/Negative matrix & target nucleic acid | 1 rep |
| Somatic cells counts – milk
JGKPT34 |
6 x preserved milk
(20 ml) |
SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing | Test immediately upon receipt | Somatic cells counting device | R.E.F 34-35
(supplied) |
Counts | 3 rep |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT47- JGKPT50 (Virus isolation) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 20-21th of March 2019. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
|
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FD: Freeze-Dried