PT Instructions
19 February 2018 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
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Scheme reference |
Samples | Samples reconstitution | Testing timeframe |
Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type |
Identification (as per the labels) |
Storage | |||||||
|
JGKPT01 Antimicrobial susceptibility |
Animal sp.: Pig 2 FD cultures (clinical pathogens) |
ATB01C: Swab in Amies ATB02C: Freeze-dried |
2-8oC |
Swab: ready to use FD: Add 1 ml sterile DH2O to the vial |
Test immediately after reconstitution Observe deadline for submission of results |
Disc diffusion technique |
R.E.F 1.2 (supplied) |
Zone Ø in millimeters. Results: Sensitive (S), Intermediate (I), Resistance (R) |
Culture ID: 1 Susceptibility: Supplied discs: 3 Own discs: 1 (see result sheet) |
| Antimicrobial discs (x18) |
Doxycycline 30 µg (x6) Colistin sulphate 10 µg (x6) Tilmicosin 30 µg (x6) |
Discs are ready to use (Commercial discs) | |||||||
|
JGKPT03 Brucella: RBT |
6 FD bovine sera | RBT01 – RBT06 | 2-8oC | Add 1 ml sterile DH2O to the vial |
Test within 3 days of reconstitution Observe deadline for submission of results |
RBT |
R.E.F 3.5 (supplied) |
Positive/Negative |
1
|
|
JGKPT04 Brucella: CFT |
6 FD bovine sera | CFT01 – CFT06 | 2-8oC | Add 1 ml sterile DH2O to the vial |
Test within 3 days of reconstitution Observe deadline for submission of results |
CFT (DAFF approved SOP for SA Labs) |
R.E.F 3.5 (supplied) |
CFTIU/ml | 2 |
|
JGKPT05 Brucella: MRT |
4 Preserved cow’s milk | CAM01 – CAM04 | 2-8oC |
NA Ready to process |
Preferably immediately but no later than 5 days after dispatch | MRT |
R.E.F 3.5 (supplied) |
Positive/Negative | 1 |
|
JGKPT06 Brucella: Culture & Identification |
3 FD cultures | CAC01 – CAC03 | 2-8oC |
Add sterile D.H2O to the vials as follows: Sample 1: 0.5 ml Samples 2 & 3: 1 ml |
Test immediately after reconstitution in BSL 2+ |
Conventional microbiology and Molecular technique |
R.E.F 06 (supplied) |
Presence/Absence: Brucella abortus Strain: Field or vaccine |
1 |
| Slides | 3 unstained smears |
Slide 1: x 3 Slide 2: x 3 Slide 3: x 3 |
Room To Dust-free |
Proceed with staining technique as per Lab SOP | When and as convenient, but before submission deadline | Stamp’s staining technique |
R.E.F 06 (supplied) |
Positive/Negative for Brucella | 1 |
|
JGKPT36-38 Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC |
NA Ready to process |
Preferably immediately but no later than 5 days after dispatch | Conventional microbiology |
R.E.F 61.4 (supplied) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 |
|
JGKPT37 & 39 Trichomonas and Campylobacter ID by PCR |
Molecular technique | Presence/Absence: Trichomonas foetus | 1 | ||||||
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Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 12th of March 2018. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
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FD: Freeze-Dried