PT Instructions

17 September 2018 Run

PURPOSE

To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1

INSTRUCTIONS

Scheme Description and Code Samples Samples  reconstitution Testing timeframe Approved test methods Sheet to be used for submission of results to JGK Unit of measurement (Results) Number of test replicates per sample
Type Identification

(as per the labels)

Storage
Avian influenza virus

(Virus isolation)

JGKPT47

3 x frozen virus suspensions

(1 ml)

AIVI01 – AIVI03 Keep frozen

-86oC

Ready to process Long term if kept frozen Virus propagation in EE R.E.F 47.50

(supplied)

Presence/Absence of target virus 1 rep
Avian influenza virus

(PCR)

JGKPT48

3 x frozen virus suspensions

(1 ml)

AIPCR01 – AIPCR03 Keep frozen

-86oC

Ready to process Long term if kept frozen PCR Presence/Absence of target nucleic acid 1 rep
Avian influenza

(Antibodies)

(H5/H6/H7)

JGKPT42

And

Newcastle disease

(Antibodies)

JGKPT44

6 x FD sera

Note:

These samples are for both AI and NDV

Test only for conditions you are registered for

AI/NDV01 – AI/NDV06 2-8oC Add sterile D.H2O

 

Sample 1: 0.8 ml

Sample 2: 1 ml

Sample 3: 1 ml

Sample 4: 0.8 ml

Sample 5: 1 ml

Sample 6: 1 ml

Test immediately after reconstitution ELISA & HA/HI tests R.E.F 42-46

(supplied)

ELISA: ODs & titres

 

HI: Titres

ELISA: 2 rep

HI: 2 rep

Bovine viral diarrhoea JGKPT40

And

Fasciola hepatica (Antibodies)

JGKPT41

5 x bovine sera

(liquid, 1 ml)

BVD/FAS01 – BVD/FAS05 2-8oC Ready to process Test within 5 days of dispatch ELISA R.E.F 40-41

(supplied)

ELISA ODs 2 rep
Infectious bronchitis virus (Antibodies)

JGKPT43

Mycoplasma gallisepticum – chicken (Antibodies)

JGKPT46

Salmonella enteritidis

(Antibodies)

JGKPT45

6 x FD sera

Note:

These samples are for IBV, MG and SE

Test only for conditions you are registered for

IBV/MG/SE01 – IBV/MG/SE06 2-8oC Add sterile D.H2O

 

Sample 1: 1 ml

Sample 2: 1 ml

Sample 3: 1 ml

Sample 4: 0.5 ml

Sample 5: 1 ml

Sample 6: 1 ml

Test immediately after reconstitution IBV: ELISA R.E.F 42-46

(supplied)

ELISA ODs

 

RSPA: Positive/Negative

ELISA: 2 rep RSPA: 1 rep
MG: RSPA/ELISA
SE: RSPA/ELISA
Newcastle disease virus (Virus isolation)

JGKPT49

3 x frozen virus suspensions

(1 ml)

NDVI01 – NDVI03 Keep frozen

-86oC

Ready to process Long term if kept frozen Virus propagation in EE R.E.F 47-50

(supplied)

Positive/Negative target virus 1 rep
Newcastle disease virus (PCR)

JGKPT50

3 x frozen virus suspensions

(1 ml)

NDPCR01 – NDPCR03 Keep frozen

-86oC

Ready to process Long term if kept frozen PCR Positive/Negative target nucleic acid 1 rep
Salmonella in Poultry

(Agent ID)

JGKPT10

3 x FD cultures SAP01 – SAP03 2-8oC Add 0.5 ml sterile D.H2O to each vial Test immediately after reconstitution Culturing / microbiological R.E.F 10-11

(supplied)

Present/Absent

PLUS

ID of the salmonella isolate

1 rep
Salmonella in Mammals

(Agent ID)

JGKPT11

3 x FD cultures SAM01 – SAM03 2-8oC Add 0.5 ml sterile D.H2O to each vial Test immediately after reconstitution Culturing / microbiological 1 rep
Treatment of samples

No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.

 

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

 

Specific environmental conditions required for the participant to conduct tests

JGKPT47- JGKPT50 (Virus isolation) must be handled in a biological safety cabinet class 2+

 

Results recording

Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

 

Submission of PT results to JGK

All results must be submitted on or before the 15th of October 2018. Any results received after this date will not be included in the analysis.

 

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 

Return of the proficiency test items

None

 

FD: Freeze-Dried