PT Instructions
14 May 2018 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme
reference |
Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification
(as per the labels) |
Storage | |||||||
| JGKPT01
Antimicrobial susceptibility (clin. pathogens) |
FD cultures | ATB01/C: Pig – Respiratory
ATB02/C: Cattle – Urinary |
2-8oC | Add 1 ml sterile DH2O to the vial | Test immediately after reconstitution
|
Disc diffusion technique | R.E.F 1.2
(supplied) |
Zone Ø in millimeters.
Report reactions as: Sensitive (“S”), Intermediate (“I”), Resistance (“R”) |
Culture ID: 1 repl
Susceptibility: JGK discs: 3 repl. Lab discs: 1 repl. (see result sheet) |
| Antimicrobial discs (x18) | Lincomycin 10 µg (x6)
Oxacillin 1 µg (x6) Neomycin 10 µg (x6) |
Discs are ready to use (Commercial discs) | |||||||
| JGKPT03
Brucella: RBT
|
6 FD bovine sera | These samples are for Labs that perform RBT only
RBT01 – RBT06 |
2-8oC | Add 0.5 ml sterile DH2O to the vial | Test within 2 days of reconstitution
|
RBT only | R.E.F 3.5
(supplied) |
Positive/Negative RBT | 1 repl.
|
| JGKPT03/04
Brucella: RBT & CFT |
6 FD bovine sera | These samples are for Labs that perform both RBT and CFT
RBT/CFT01 – RBT/CFT06 |
2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution
|
RBT and CFT
(CFT: DAFF approved SOP for SA Labs) |
R.E.F 3.5
(supplied) |
RBT: Positive/Negative CFT: IU/ml | RBT: 1 repl.
CFT: 2 repl. |
| JGKPT05
Brucella: MRT |
4 cow’s milk | CAM01 – CAM04 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 5 days after dispatch | MRT | R.E.F 3.5
(supplied) |
Positive/Negative MRT | 1 repl. |
| JGKPT06
Brucella: Culture & Identification |
3 bovine milk | CAC01 – CAC03 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 5 days after dispatch | Conventional microbiology and
Molecular technique |
R.E.F 06
(supplied) |
Presence/Absence: Brucella abortus
Strain: Field or vaccine |
1 repl. |
| Slides | 3 unstained smears | Slide 1: x 3
Slide 2: x 3 Slide 3: x 3 |
Room To
Dust-free |
Proceed with staining technique as per Lab SOP | When and as convenient, but before submission deadline | Stamp’s staining technique | R.E.F 06
(supplied) |
Positive/Negative for Brucella | 1 repl. |
| JGKPT10
Salmonella – Poultry |
3 FD cultures | SAP01 – SAP03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution
|
Conventional microbiology | R.E.F 8.11
(supplied) |
Presence/Absence: Salmonella
Full ID of the salmonella isolate |
1 repl. |
| JGKPT11
Salmonella – Mammals |
3 FD cultures | SAM01 – SAM03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology | R.E.F 8.11
(supplied) |
Presence/Absence: Salmonella
Full ID of the salmonella isolate |
1 repl. |
| JGKPT12
Cattle Disease Scenario |
3 FD cultures | BAC01 – BAC03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology | R.E.F 12.7
(supplied) |
Full ID of the bacteria isolated | 1 repl. |
| JGKPT13
Chicken Disease Scenario |
3 FD cultures | BACH01 – BACH03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology | R.E.F 12.7
(supplied) |
Full ID of the bacteria isolated | 1 repl. |
| JGKPT16
Pig Disease Scenario |
3 FD cultures | BAP01 – BAP03 | 2-8oC | Add 1 ml sterile DH2O to the vial | Test within 2 days of reconstitution | Conventional microbiology | R.E.F 12.7
(supplied) |
Full ID of the bacteria isolated | 1 repl. |
| JGKPT32-33
Aflatoxin and Fumonisins |
3 maize flower | MYC01 – MYC03 | 2-8oC | NA
Ready to process |
Test within 2 days of dispatch | ELISA, Chromatography | R.E.F 32.3
(supplied) |
Aflatoxin: B1/B2/G1/G2
Fumonisin: B1/B2 |
2 repl. |
| JGKPT36-38
Trichomonas and Campylobacter ID by Culture |
5 Cultures in transport media | SW01 – SW05 | 2-8oC | NA
Ready to process |
Preferably immediately but no later than 5 days after dispatch | Conventional microbiology | R.E.F 36.9
(supplied) |
Presence/Absence: Trichomonas foetus, Campylobacter fetus | 1 repl. |
| JGKPT37 & 39
Trichomonas and Campylobacter ID by PCR |
Molecular technique | Presence/Absence: Trichomonas foetus | 1 repl. | ||||||
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests All cultures must be handled in a biological safety cabinet. Minimum requirement for JGKPT06 (Brucella Culture): class 2+
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 04th of June 2018. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None |
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FD: Freeze-Dried