PT Instructions
20 August 2018 Run
PURPOSE
To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1
INSTRUCTIONS
| Scheme Description and Code | Samples | Samples reconstitution | Testing timeframe | Approved test methods
|
Sheet to be used for submission of results to JGK | Unit of measurement (Results) | Number of test replicates per sample | ||
| Type | Identification | Storage | |||||||
| Antibiogram – mastitis pathogens
JGKPT02 |
2 x cow’s milk
(10 ml) |
ATB01M – ATB02M | 2-8oC | Ready to process | Culturing of bacteria should be done Immediately upon receipt of samples | Culturing: clin. micro
Susceptibility: Kirby-Bauer / Disc diffusion |
R.E.F 02
(supplied) |
Zone Ø in millimeters
Results expressed as: S, I, R |
Culture: 1 rep
Susceptibility: 3 rep supplied discs; 1 rep own discs |
| 18 x antibiotic discs | 6 x Penicillin 10
6 x Erythromycin 15, 6 x Tetracycline 30 |
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| Antimicrobial residues – meat
JGKPT24-26 |
4 x pieces of meat
(>25 g) |
RES01F – RES04F | 2-8oC | Ready to process | Test within 5 days of dispatch | Rapid test
Microbiology Chromatography |
R.E.F 24-26
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| Antimicrobial residues – milk
JGKPT21-23 |
4 x milk
(liquid, 4ml) |
RES01M – RES04M | 2-8oC | Ready to process | Test within 5 days of dispatch | Rapid test
Microbiology Chromatography |
R.E.F 21-23
(supplied) |
Presence/Absence of antimicrobials | 1 rep |
| Food hygiene – meat (Detection)
JGKPT27-31 |
3 x pieces of meat
(>25 g) |
FH01 – FH03 | 2-8oC | Ready to process | Test within 5 days of dispatch | Microbiology (conventional & automated) | R.E.F 27-31
(supplied) |
Presence/Absence of target bacteria | 1 rep |
| Mastitis pathogens – milk
JGKPT35 |
2 x cow’s milk
(4 ml) |
MAST01 – MAST02 | 2-8oC | Ready to process | As for Antibiogram. Test immediately | Culture & ID | R.E.F 34-35
(supplied) |
Target mastitis bacteria | 1 rep |
| Milk hygiene: TBC, Coliforms and E. coli count
JGKPT18-20 |
5 x FD milk
(6 ml) |
MH01 – MH05 | 2-8oC | Add 10 ml sterile DH2O to the vial
Mix well and test |
Test immediately after reconstitution | 3M Petri Films | R.E.F 18-20 | For each samples test:
TBC Coliforms E. coli |
2 rep |
| Somatic cells counts – milk
JGKPT34 |
6 x preserved milk
(20 ml) |
SCC01 – SCC06 | 2-8oC | Mix thoroughly before testing | Test within 5 days of dispatch | Somatic cells counting device | R.E.F 34-35
(supplied) |
Counts | 3 rep |
| Mycotoxins – maize
JGKPT32 – 33 |
3 x maize meal
(100 g) |
MYC 01 – 03 | 2-8oC | Ready to process | Test within 5 days of dispatch | ELISA
Chromatography Other (specify) |
R.E.F 32-33
(supplied) |
Aflatoxin and Fumonisin
Presence / absence Quantification |
Qualitative: 1
Quantitative: 1 (average) |
| Treatment of samples
No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.
Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.
Specific environmental conditions required for the participant to conduct tests None
Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.
Submission of PT results to JGK All results must be submitted on or before the 10th of September 2018. Any results received after this date will not be included in the analysis.
Enquiries For any inquiry, please use the contact details provided above to contact JGK
Return of the proficiency test items None
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FD: Freeze-Dried