PT Instructions

28 July 2020 Run

PURPOSE

To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1

INSTRUCTIONS

Scheme

reference

Samples Samples  reconstitution Testing timeframe Approved test methods

 

Sheet to be used for submission of results to JGK Unit of measurement (Results) Number of test replicates per sample
Type Identification

(as per the labels)

Storage
JGKPT01

Antimicrobial susceptibility

(Clin. pathogens)

2 FD cultures ATB01/C – Dog

ATB02/C – Pig

2-8oC Add 1 ml sterile DH2O to the vial. Mix Test without delay after reconstitution of the samples Disc diffusion technique (Qualitative - Kirby Bauer) R.E.F 1.0

(supplied)

Zone Ø in millimeters.

Results: Sensitive (S), Intermediate (I), Resistance (R)

Culture ID: 1

Susceptibility:

Supplied discs: 3

(as per result sheet)

Antimicrobial discs (x18) Chloramphenicol 30 µg (6)

Gentamycin 10 µg (6)

Polymixin B 300 units (6)

Discs are ready to use (Commercial discs)
JGKPT03

Brucella: RBT

6 FD cattle sera

(samples for Labs testing for RBT only)

RBT01 – RBT06 2-8oC Add 0.5 ml sterile DH2O to the vial. Mix well before testing Test without delay after reconstitution of the samples RBT R.E.F 3-5

(supplied)

Positive/Negative 1
JGKPT04

Brucella: CFT

6 FD cattle sera (samples for Labs testing for both RBT and CFT) RBT/CFT01 – RBT/CFT06 2-8oC Sample 2: Add 0.5 ml sterile DH2O to the vial. All the other samples, add 1 ml. Mix well Test without delay after reconstitution of the samples CFT

(DAFF approved SOP for SA Labs)

R.E.F 3-5

(supplied)

CFTIU/ml 2
JGKPT05

Brucella: MRT

4 bulk tank milk CAM01 – CAM04 2-8oC NA

Ready to process

Preferably immediately but no later than 3 days from date of dispatch MRT R.E.F 3-5

(supplied)

Positive/Negative 1
JGKPT06

Brucella ID: Culture & PCR

2 FD cultures

2 milk

CAC01 & CAC04: Milk

CAC02 & CAC03: FD

2-8oC FD: Add 1 ml sterile DH2O to the vial. Mix well before testing

Milk: Ready to process

FD: Test without delay after reconstitution of the samples Conventional microbiology and

Molecular  technique

R.E.F 06

(supplied)

Presence/Absence: Brucella abortus

Strain:

Field or vaccine

1
JGKPT07

Brucella Slides

3 unstained smears Slide 1: x 3

Slide 2: x 3

Slide 3: x 3

Room To

Dust-free

Proceed with staining technique as per Lab SOP When and as convenient, but before submission deadline Stamp’s staining technique R.E.F 06

(supplied)

Positive/Negative for Brucella 1
JGKPT09

Salmonella Poultry

2 FD cultures

1 Swab

SAP01 & SAP03: FD

SAP02: Swab

2-8oC Add 0.5 ml sterile DH2O to the vial. Mix well Test without delay after reconstitution of the samples Conventional microbiology and

Molecular  technique

R.E.F 08-10

(supplied)

Positive/Negative for Salmonella + full ID of target salmonella 1
JGKPT10

Salmonella mammals

3 FD cultures SAM01 – SAM03 2-8oC Add 0.5 ml sterile DH2O to the vial. Mix well Positive/Negative for Salmonella + full ID of target salmonella 1
JGKPT11

Diseases of Chicken

3 FD cultures BAC01/CH – BAC03/CH 2-8oC Add 0.5 ml sterile DH2O to the vial. Mix well Test immediately upon reconstitution of the samples Conventional microbiology and

Molecular  technique

R.E.F 08-10

(supplied)

Full ID of the bacteria isolated 1
JGKPT35 & 37

Trichomonas and Campylobacter ID by Culture

5 Cultures in transport media SW01 – SW05 2-8oC NA

Ready to process

Preferably immediately but no later than Friday 31st July for culture (samples were prepared on Sunday 26 July) Conventional microbiology R.E.F 35-38

(supplied)

Presence/Absence: Trichomonas foetus, Campylobacter fetus 1
JGKPT36 & 38

Trichomonas and Campylobacter ID by PCR

Molecular  technique Presence/Absence: Trichomonas foetus 1
 

Treatment of samples

No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.

 

 

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

 

 

Specific environmental conditions required for the participant to conduct tests

JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+

 

 

Results recording

Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

 

 

Submission of PT results to JGK

All results must be submitted on 12th of August 2020. Any results received after this date will not be included in the analysis.

 

 

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 

 

Return of the proficiency test items

None

 

FD: Freeze-Dried