03 August 2020 Run – JGK Lab AfriQA

Scheme Description and Code	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Scheme Description and Code	Type	Identification (as per the labels)	Storage	Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Antibiogram – mastitis pathogens JGKPT02	2 x FD	ATB01M – ATB02M	2-8^oC	Add 1 ml sterile DH₂O to each vial	Culturing of bacteria should be done Immediately upon reconstitution	Culturing: micro Susceptibility: Kirby-Bauer / Disc diffusion (Qualitative)	R.E.F 02 (supplied)	Zone Ø in millimeters (Results: S, I, R)	Culture: 1 rep Susceptibility: 3 replicates (supplied discs)
Antibiogram – mastitis pathogens JGKPT02	18 x antibiotic discs	6 x Tetracycline 30 µg 6 x Ticarcillin/Clavulanic acid 85 µg 6 x Amikacin 30 µg	2-8^oC	Ready to process			R.E.F 02 (supplied)	Zone Ø in millimeters (Results: S, I, R)
Antimicrobial residues – meat JGKPT23-25	4 x pieces of meat	RES-01-F – RES-04-F	2-8^oC	Ready to process	Test immediately upon receipt	Rapid test Microbiology Chromatography	R.E.F 23-25 (supplied)	Presence/Absence of antimicrobials ID of the antibiotic	1 rep
Antimicrobial residues – milk JGKPT20-22	4 x milk (liquid)	RES-01-M – RES-04-M	2-8^oC	Ready to process	Test immediately upon receipt	Rapid test Microbiology Chromatography	R.E.F 20-22 (supplied)	Presence/Absence of antimicrobials ID of the antibiotic	1 rep
Food hygiene – meat (Detection) JGKPT25-30	3 x FD	FH01 – FH03	2-8^oC	Add 1 ml sterile DH₂O to each vial	Test immediately after reconstitution	Microbiology (conventional &	R.E.F 25-30 (supplied)	Presence/Absence of target bacteria	1 rep
Mastitis pathogens – milk JGKPT34	2 x FD	MAST01 – MAST02	2-8^oC	Add 1 ml sterile DH₂O to each vial	Test immediately after reconstitution	Culture & ID	R.E.F 33-34 (supplied)	Target bacteria	1 rep
Milk hygiene: TBC, Coliforms and E. coli counts JGKPT17-19	4 x FD milk (10 ml)	MH01 – MH04	2-8^oC	Add 10 ml sterile DH₂O to each vial. Vortex /mix thoroughly before testing NB. DH₂O must adjust to room T^o before use	Let the reconstituted milk stand for 30 min at ambient temperature before testing.	3M Petri Films Conventional technique	R.E.F 17-19	Counts	2 rep
Aflatoxins / Fumonisins JGKPT31-32	3 x maize meal	MYC01 – MYC03	2-8^oC	Ready to process	Test immediately upon receipt	Chromatography	R.E.F 31-32	Presence & quantification (ppb) of Aflatoxins and Fumonisins	1 rep.
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests None Results recording Only the supplied excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on or before the 19^th of August 2020. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None