reference

(as per the labels)

Antimicrobial susceptibility

(Clin. Pathogens)

ATB02/C – Dog isolate

Remove seals and bungs from the vials and add 750 µl sDH₂O to each vial.

Allow the pellet to dissolve completely. If necessary, tilt the vial at various angles to ensure all materials are dissolved. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (per the lab’s SOP)—streak with a sterile loop to facilitate discrete colonies.

Proceed with Antibiogram testing as per the Lab’s SOP

Preferably, test the samples immediately upon receipt and reconstitution.

For short-term storage, keep the samples between 2 °C and 8 °C.

(Kirby Bauer)

(available on www.jgklab.co.za)

Report results as:

Sensitive (S), Intermediate (I), Resistance (R)

Susceptibility

(supplied discs): 3 as per the result sheet

(x 18+)

Oxytetracycline 30 µg; Oxoid 6265879; exp. 14/05/2028.

Penicillin G 10 IU; Oxoid 6220791; exp. 21/01/2028.

Brucella: RBT

(Samples for Labs doing RBT only)

Allow sera and sDH₂O to adjust to room temperature.

Remove seals and bungs, and add sDH₂O as follows:

Sample/vial 1: 300 µl

Sample/vial 2: 500 µl

Sample/vial 3: 500 µl

Sample/vial 4: 350 µl

Sample/vial 5: 500 µl

Sample/vial 6: 550 µl

Do not add cold water.

There must be no clots in the serum when tested

(available on www.jgklab.co.za)

Or

Negative

Brucella: RBT and CFT

(Samples for Labs doing both RBT and CFT)

Allow sera and sDH₂O to adjust to room temperature.

Remove seals and bungs and add sDH₂O to samples as follows:

Samples 1, 2, 3, 4, 5: add 500 µl.  Sample 6: add 550 µl.

Do not add cold water.

There must be no clots in the serum when tested

CFT: R.E.F 04

(available on www.jgklab.co.za)

Positive (low, medium, high)

Or

Negative

Option 1: Endpoint reaction (e.g., 1/4 ++++, 1/128+)

Option 2: Titer in CFTIU (e.g., 24, 480)

2

Brucella: MRT

(not preserved)

Ready to process

(available on www.jgklab.co.za)

Negative

Brucella: Culture & Identification

Remove seals and bungs from the vials and sDH₂O as follows: 1 ml to sample 1, 750 µl to sample 2, and 500 µl to sample 3.

Allow the pellet to dissolve completely. If necessary, tilt the vial at various angles to ensure all materials are dissolved. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (per the lab’s SOP)—streak with a sterile loop to facilitate discrete colonies.

Preferably, test the samples immediately upon receipt.

For short-term storage, keep the samples between 2 °C and 8 °C.

and

Molecular technique

(available on www.jgklab.co.za)

And

Strain:

Field or vaccine

Clinical Microbiology:

Salmonella Poultry

Allow cultures and sDH₂O to adjust to room temperature.

Remove seals and bungs and add 750 µl sDH₂O to each Sample/vial.

Allow the pellet to dissolve completely. If necessary, tilt the vial at various angles to ensure all materials are dissolved. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (per the lab’s SOP)—streak with a sterile loop to facilitate discrete colonies.

Preferably, test the samples immediately upon receipt.

For short-term storage, keep the samples between 2 °C and 8 °C.

and

Molecular technique

(available on www.jgklab.co.za)

Clinical Microbiology:

Salmonella Mammals

Trichomonas and Campylobacter ID by Culture

Ready to process

(available on www.jgklab.co.za)

Trichomonas foetus/ Campylobacter fetus

Trichomonas and Campylobacter ID by PCR

Treatment of samples

No special treatment should be given to the PT samples. They [PT samples] should be handled in the same manner as all the other samples routinely tested in the laboratory, including TAT

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per the laboratory standard operating procedure. Unused materials and waste must be removed as per laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

Specific environmental conditions required for the participant to conduct tests:

JGKPT06 (Brucella Culture) must be handled in a biological safety cabinet class 2+

Results recording

Only the supplied Excel “Result entry forms” [R.E.F] should be used to capture the PT results. The R.E.F are available online (www.jgklab.co.za) and can be downloaded under “PUBLICATIONS >> RESULT SHEET”. All the fields of the R.E.F must be filled.

Submission of PT results to JGK

All results must be submitted on Wednesday, 11^th of March 2026. Any results received after this date will not be included in the analysis.

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

Return of the proficiency test items

None