PT Instructions

WEEK 20: 12 MAY 2025 RUN

PURPOSE

To provide for detailed instructions for participants in compliance with the requirements of ISO 17043, clause 4.6.1

INSTRUCTIONS

Scheme Description and Code Samples Samples reconstitution Testing timeframe Approved test methods Sheet to be used for submission of results to JGK Unit of measurement (Results) Number of test replicates per sample
Type Identification

(as per the labels)

 Storage
Antibiogram – Mastitis pathogens

JGKPT02

 2 x FD cultures ATB 01/M – Dairy cow

ATB 02/M – Dairy cow

 2-8oC Allow cultures and sDH2O to adjust to room temperature.

Remove seals and bungs from the vials and add 450 µl of sDH2O to each vial.

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies

 These samples are not intended to be stored.

Test the samples immediately upon receipt and reconstitution

 Culturing: Micro

Susceptibility: Disc diffusion (Kirby-Bauer) / Qualitative

 

 

 R.E.F 02

(supplied)

 Zone Ø in millimeters.

Report results as:

Sensitive (S), Intermediate (I), Resistance (R)

 Culture: 1 rep

Susceptibility:

3 replicates (supplied discs)
Antimicrobial discs

(x 18)

 Pirlimycin 2 µg; Oxoid 3777591; exp. 01/2025

Penicillin 10 IU; Oxoid 6220791; Exp. 21/01/2028

Neomycin 30 µg; Oxoid 3747259; Exp. 10/2026

 Commercial discs:

Ready to use
Antimicrobial residues – Meat

JGKPT23-25

 4 x Fresh samples

(chicken liver)

 RES 01/F – RES 04/F 2-8oC Fresh samples: Ready to process Test immediately upon receipt Rapid test

Microbiology

Chromatography

 R.E.F 23-25

(supplied)

 Presence/Absence of antimicrobials

 

ID of the antibiotic

 1 rep
Antimicrobial residues – Milk

JGKPT20-22

 4 x Fresh cow’s milk RES 01 – RES 04 2-8oC N/A

Ready to process

 Test immediately upon receipt Rapid test

Microbiology

Chromatography

 R.E.F 20-22

(supplied)

 Presence/Absence of antimicrobials

 

Name of the antibiotic

 1 rep
Food hygiene – Detection

JGKPT25-30

 5 x FD samples

5 x Amies swabs

 

(Identical samples)

 FH 01 – FH 05 2-8oC FD samples:

Allow cultures and sDH2O to adjust to room temperature.

Remove seals and bungs from the vials and add 450 µl of sDH2O to each vial.

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies

 

Swabs: ready to process

 

NB:

Test both the FD & Amies swabs

 Test immediately upon receipt/ reconstitution Microbiology (conventional & PCR) R.E.F 25-30

(supplied)

 Presence/absence of the target bacteria 1 rep
4 x FD samples FH 01/Counts – FH 04/Counts 2-8oC Allow samples to adjust to room temperature. Add 6 ml of preheated (37 °C) sterile DH2O to each jar. Mix the content thoroughly on a vortex until there are no clumps These samples are not intended to be stored.

Test the samples immediately upon receipt and reconstitution

 Counts TBC: 2 reps

Coliforms: 2 reps

E. coli: 2 reps
Mastitis pathogens JGKPT34 2 x FD cultures MAST 01 – MAST 02 2-8oC Allow cultures and sDH2O to adjust to room temperature.

Remove seals and bungs from the vials and add 450 µl of sDH2O to each vial.

Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies

 These samples are not intended to be stored.

Test the samples immediately upon receipt and reconstitution

 Culture & ID R.E.F 33-34

(supplied)

 Target bacteria 1 rep
Milk hygiene: TBC, Coliforms, and E. coli counts

JGKPT17-19

 4 x FD milk (bovine) MH 01 – MH 04 2-8oC Add 6 ml sterile DH2O

to each jar

NB:

Do not use cold water. Samples must adjust to room temperature for ≥ 30 min before adding sD.H2O.

D.H2O must be at 37oC in a water bath for ≥30 min before use. Mix the content thoroughly on a vortex until there are no clumps.

 These samples are not intended to be stored.

Test the samples immediately upon receipt and reconstitution

 3M Petri Films

Conventional technique

 R.E.F 17-19 Counts TBC: 2 reps

Coliforms: 2 reps

E. coli: 2 reps
BVD & Fasciola

JGKPT39-40

 5 x FD sera (bovine) BVD/FAS01 – BVD/FAS05 2-8oC Allow samples and sDH2O to adjust to room temperature. Add 350 µL of sDH2O to vials 1, 2, 3, and 300 µL of sDH2O to vials 4 and 5 These samples are not intended to be stored.

Test the samples immediately upon receipt and reconstitution

 ELISA R.E.F 39-45 Elisa ODs 2 reps.
Avian influenza - Abs

JGKPT41

 6 x FD sera (chicken) AI 01 – AI 06 2-8oC Allow samples and sDH2O to adjust to room temperature. Sample volumes: 2 volumes are provided: a small volume for labs doing ELISA or HI test only, a big volume for labs doing both ELISA & HI (there is a red mark in the top right of the labels).

 

Add sDH2O as follows:

Sample 1: 250 µL/500 µL

Sample 2: 250 µL/500 µL

Sample 3: 250 µL/500 µL

Sample 4: 300 µL/500 µL

Sample 5: 300 µL/500 µL

Sample 6: 300 µL/500 µL

 Test immediately upon receipt/

reconstitution

 ELISA

HA/HI

 R.E.F 39-45 ODs

Titers

 2 reps
Infectious bronchitis virus – Abs

  JGKPT42

 6 x FD sera (chicken) IBV 01 – IBV 06 2-8oC Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:

Sample 1: 300 µL

Sample 2: 250 µL

Sample 3: 250 µL

Sample 4: 300 µL

Sample 5: 300 µL

Sample 6: 300 µL

 Test immediately upon receipt/

reconstitution

 ELISA R.E.F 39-45 ODs

Titers

 2 reps
Newcastle disease – Abs JGKPT43 6 x FD sera (poultry) NDV 01 – NDV 06 2-8oC Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:

Sample 1: 300 µL

Sample 2: 300 µL

Sample 3: 250 µL

Sample 4: 300 µL

Sample 5: 250 µL

Sample 6: 300 µL

 Test immediately upon receipt/

reconstitution

 ELISA

HA/HI

 R.E.F 39-45 ODs

Titres

 2 rep
Salmonella enteritidis – Abs

JGKPT44

 6 x FD sera (poultry) SE 01 – SE 06 2-8oC Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:

Sample 1: 300 µL

Sample 2: 300 µL

Sample 3: 250 µL

Sample 4: 300 µL

Sample 5: 300 µL

Sample 6: 300 µL

 Test immediately upon receipt/

reconstitution

 ELISA

RPAT

 R.E.F 39-45 Positive / Negative 1 rep
Mycoplasma gallisepticum - Abs

JGKPT45

 6 x FD sera (poultry) MG 01 – MG 06 2-8oC Allow samples and sDH2O to adjust to room temperature. Add sDH2O to the vials as follows:

Sample 1: 350 µL

Sample 2: 300 µL

Sample 3: 300 µL

Sample 4: 300 µL

Sample 5: 200 µL

Sample 6: 250 µL

 Test immediately upon receipt/

reconstitution

 ELISA

RPAT

 R.E.F 39-45 Positive / Negative 1 rep
Treatment of samples

No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory.

 

Handling and safety requirements

Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per the laboratory standard operating procedure. Unused materials and waste must be removed as per the laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company.

 

Specific environmental conditions required for the participant to conduct tests:

None

 

Results recording

Only the supplied Excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F.

 

Submission of PT results to JGK

All results must be submitted on or before Wednesday, the 4th of June 2025. Any results received after this date will not be included in the analysis.

 

Enquiries

For any inquiry, please use the contact details provided above to contact JGK

 

Return of the proficiency test items

None

FD: Freeze-Dried