WEEK 32: 04 AUGUST 2025 RUN – JGK Lab AfriQA

Scheme Description and Code	Samples			Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Scheme Description and Code	Type	Identification (as per the labels)	Storage	Samples reconstitution	Testing timeframe	Approved test methods	Sheet to be used for submission of results to JGK	Unit of measurement (Results)	Number of test replicates per sample
Antibiogram – Mastitis pathogens JGKPT02	2 x FD cultures 2 x Amies transport swabs (BKP samples)	ATB 01/M – Dairy cow ATB 02/M – Dairy cow	2-8^oC	Allow cultures and sDH₂O to adjust to room temperature. Remove seals and bungs from the vials and add sDH₂O to the vials as follows: Vial 1 (ATB 01/M): 450 µl, Vial 2 (ATB 02/M): 650 µl. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	Culturing: Micro Susceptibility: Disc diffusion (Kirby-Bauer) / Qualitative	R.E.F 02 (supplied)	Zone Ø in millimeters. Report results as: Sensitive (S), Intermediate (I), Resistance (R)	Culture: 1 rep Susceptibility: 3 replicates (supplied discs)
Antibiogram – Mastitis pathogens JGKPT02	Antimicrobial discs (x 18)	Oxytetracycline 30 µg; Oxoid 6265879; exp. 14/05/2028 Erythromycin 15 µg; Oxoid 6205816; Exp. 13/12/2027 Oxacillin 1 µg; Oxoid 3646846; Exp. 27/03/2027	2-8^oC	Commercial discs: Ready to use			R.E.F 02 (supplied)
Antimicrobial residues – Meat JGKPT23-25	4 x Fresh samples (chicken liver)	RES 01/F – RES 04/F	2-8^oC	Fresh samples: Ready to process	Test immediately upon receipt	Rapid test Microbiology Chromatography	R.E.F 23-25 (supplied)	Presence/Absence of antimicrobials ID of the antibiotic	1 rep
Antimicrobial residues – Milk JGKPT20-22	4 x FD cow’s milk	RES 01 – RES 04	2-8^oC	Allow samples and sterile sDH₂O to reach room temperature. Remove only the seals and inject 2 mL of sDH₂O into the vial. Mix the contents thoroughly on a vortex until no clumps remain. Remove the bungs and proceed with the test.	Test immediately upon receipt and reconstitution.	Rapid test Microbiology Chromatography	R.E.F 20-22 (supplied)	Presence/Absence of antimicrobials Name of the antibiotic	1 rep
Food hygiene – Detection JGKPT25-30	5 x FD samples 5 x Amies swabs (Identical samples)	FH 01 – FH 05	2-8^oC	FD samples: Allow cultures and sDH₂O to reach room temperature. Remove seals and bungs from the vials and add 650 µl of sDH₂O to each vial. Allow the pellet to dissolve completely. Immediately, saturate a sterile swab with the reconstituted sample and inoculate 1/3 of the agar/culture plates (plates as per your lab’s SOP). Using a sterile loop streak to facilitate discrete colonies Swabs: ready to process NB: Test both the FD & Amies swabs	Test immediately upon receipt/ reconstitution	Microbiology (conventional & PCR)	R.E.F 25-30 (supplied)	Presence/absence of the target bacteria (Salmonella species, Escherichia coli, and Listeria monocytogenes)	1 rep
Food hygiene – Enumeration	4 x FD samples	FH 01/Counts – FH 04/Counts	2-8^oC	Allow samples to reach room temperature. Add 5 mL of preheated (37 °C) sterile DH₂O to each jar. Mix the content thoroughly on a vortex until there are no clumps	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	Microbiology (conventional & PCR)	R.E.F 25-30 (supplied)	Counts	TBC: 2 reps Coliforms: 2 reps E. coli: 2 reps
Mastitis pathogens JGKPT34	2 x cow’s milk	MAST 01 – MAST 02	2-8^oC	N/A Ready to process	Test the samples immediately upon receipt.	Culture & ID	R.E.F 33-34 (supplied)	Target bacteria	1 rep
Milk hygiene: TBC, Coliforms, and E. coli counts JGKPT17-19	4 x FD milk (bovine)	MH 01 – MH 04	2-8^oC	Add sterile DH₂O to the jars as follows: Jars 1-3: 5 mL; Jar 4: 6 mL. NB: Do not use cold water. Samples must reach room temperature for ≥ 30 min before adding sD.H₂O. D.H₂O must be at 37^oC in a water bath for ≥30 min before use. Mix the content thoroughly on a vortex until there are no clumps.	These samples are not intended to be stored. Test the samples immediately upon receipt and reconstitution	3M Petri Films Conventional technique	R.E.F 17-19	Counts	TBC: 2 reps Coliforms: 2 reps E. coli: 2 reps
Mycotoxins: Aflatoxins & Fumonisins JGKPT31-32	2 x Maize meal	MYC 01 – MYC 02	2-8^oC	N/A Ready to process	Test the samples immediately upon receipt	Chromatography and ELISA	R.E.F 31-32	Detection and quantification of Aflatoxins (in ppb) and Fumonisins (in ppm)	1 rep.
Treatment of samples No special treatment should be given to the PT samples. They [samples] should be handled in the same manner as all the other samples routinely tested in the laboratory. Handling and safety requirements Contact surfaces must be decontaminated with 70% alcohol or other suitable disinfectants. Used laboratory utensils must be cleaned and sterilized as per the laboratory standard operating procedure. Unused materials and waste must be removed as per the laboratory waste management protocol. This involves autoclaving and/or removal by an approved waste removal company. Specific environmental conditions required for the participant to conduct tests: None Results recording Only the supplied Excel “Result entry forms” [R.E.F] should be used to capture the PT results. The Participants must fill in the cover page and answer all questions before capturing the results on R.E.F. Submission of PT results to JGK All results must be submitted on or before Wednesday, the 27^th of August 2025. Any results received after this date will not be included in the analysis. Enquiries For any inquiry, please use the contact details provided above to contact JGK Return of the proficiency test items None